Histone deacetylases and SAP18, a novel polypeptide, are components of a human Sin3 complex

Cell. 1997 May 2;89(3):357-64. doi: 10.1016/s0092-8674(00)80216-0.

Abstract

An important event in gene expression is the covalent modification of histone proteins. We have found that the mammalian transcriptional repressor Sin3 (mSin3) exists in a complex with histone deacetylases HDAC1 and HDAC2. Consistent with the observation that mSin3-mediated repression of transcription involves the modification of histone polypeptides, we found that the mSin3-containing complex includes polypeptides that tether the mSin3 complex to core histone proteins. In addition, two novel mSin3-associated polypeptides, SAP18 and SAP30, were identified. We isolated a cDNA encoding human SAP18 and found that SAP18 is a component of an mSin3-containing complex in vivo. Moreover, we demonstrate a direct interaction between SAP18 and mSin3. SAP18 represses transcription in vivo when tethered to the promoter, consistent with the ability of SAP18 to interact with mSin3.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Western
  • Carrier Proteins / metabolism
  • Cell Fractionation
  • HeLa Cells / physiology
  • Histone Deacetylases / metabolism*
  • Humans
  • Mammals
  • Molecular Sequence Data
  • Multienzyme Complexes / chemistry*
  • Multienzyme Complexes / genetics
  • Multienzyme Complexes / metabolism
  • Nuclear Proteins / metabolism
  • Precipitin Tests
  • Repressor Proteins / genetics
  • Repressor Proteins / isolation & purification
  • Repressor Proteins / metabolism*
  • Retinoblastoma
  • Retinoblastoma-Binding Protein 4
  • Retinoblastoma-Binding Protein 7
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Transcription Factors / genetics*
  • Transcription Factors / isolation & purification
  • Transcription Factors / metabolism*
  • Transcription, Genetic / physiology

Substances

  • Carrier Proteins
  • Multienzyme Complexes
  • Nuclear Proteins
  • RBBP4 protein, human
  • RBBP7 protein, human
  • Rbbp4 protein, mouse
  • Repressor Proteins
  • Retinoblastoma-Binding Protein 4
  • Retinoblastoma-Binding Protein 7
  • SAP18 protein, human
  • SIN3 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Histone Deacetylases

Associated data

  • GENBANK/U96915