Regulation of the metabolism of plasma lipoproteins by apolipoprotein A-II

Biochim Biophys Acta. 1997 Apr 21;1345(3):248-58. doi: 10.1016/s0005-2760(96)00183-x.

Abstract

Mouse apolipoprotein (apo) A-II has three variants (type A, B, and C) among inbred strains. To clarify the role of ApoA-II in the metabolism of high density lipoproteins (HDL), we constructed a new congenic mouse strain (P1.R1-Apoa2b) with type B ApoA-II of the SAMR1 strain on the genetic background of the SAMP1 strain, and examined it together with another ApoA-II congenic strain (R1.P1-Apoa2c) containing type C ApoA-II of the SAMPI strain on the SAMR1 strain and the parental SAMP1 and SAMR1 strains. Genetic characterization of the congenic strains indicated that only small regions surrounding the ApoA-II gene of the parental strains had been transferred. The strains with Apoa2c had lower plasma concentrations of HDL and ApoA-II, and a smaller HDL particle size than strains with Apoa2b. We detected no significant differences in the mRNA levels of ApoA-II or in the in vitro translational efficiency of the ApoA-II mRNA among the four strains. These findings suggested that the differences in the post-translational modification or efficiency of secretion between the Apoa2b and Apoa2c protein regulates the ApoA-II concentration which in turn determines the concentration and size of HDL in mice.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animal Feed
  • Animals
  • Apolipoprotein A-II / blood
  • Apolipoprotein A-II / genetics*
  • Dietary Fats / administration & dosage*
  • Lipoproteins / blood*
  • Lipoproteins, HDL / blood
  • Lipoproteins, HDL / chemistry
  • Mice
  • Mice, Mutant Strains
  • Polymorphism, Genetic
  • Protein Biosynthesis
  • RNA, Messenger / analysis

Substances

  • Apolipoprotein A-II
  • Dietary Fats
  • Lipoproteins
  • Lipoproteins, HDL
  • RNA, Messenger