Renal cell carcinoma and normal kidney protein expression

Electrophoresis. Mar-Apr 1997;18(3-4):599-604. doi: 10.1002/elps.1150180343.

Abstract

Renal cell carcinoma (RCC), a human kidney cancer from the proximal tubular epithelium, accounts for about 3% of adult malignancies. Molecular and cytogenetic analysis have highlighted deletions, translocations, or loss of heterozygosity in the 3p21-p26, a putative RCC locus, as well as in 6q, 8p, 9pq, and 14pq. Studies on phenotypic expression of human kidney tissue and on post-translational modifications in RCC have not yet provided a marker for early renal cell carcinoma diagnosis. Current diagnostic methods do not help to detect the tumor before advanced stages. We therefore used two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) to study normal and tumor kidney tissues in ten patients suffering from RCC. A human kidney protein map in the SWISS-2DPAGE database accessible through the ExPASy WWW Molecular Biology Server was established. Of 2789 separated polypeptides, 43 were identified by gel comparison, amino acid analysis, N-terminal sequencing, and/or immunodetection. The comparison between normal and tumor kidney tissues showed four polypeptides to be absent in RCC. One of them was identified as ubiquinol cytochrome c reductase (UQCR), whose locus has elsewhere been tentatively assigned to chromosome 19p12 or chromosome 22. A second polypeptide was identified as mitochondrial NADH-ubiquinone oxido-reductase complex I whose locus is located on chromosome 18p11.2 and chromosome 19q13.3. These result suggest that the lack of UQCR and of mitochondrial NADH-ubiquinone oxidoreductase complex I expression in RCC may be caused by unknown deletions, or by changes in gene transcription or translation. It might indicate that mitochondrial disfunction plays a major role in RCC genesis or evolution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Carcinoma, Renal Cell / chemistry*
  • Carcinoma, Renal Cell / pathology
  • Electrophoresis, Gel, Two-Dimensional
  • Humans
  • Kidney Neoplasms / chemistry*
  • Kidney Neoplasms / pathology
  • Kidney Tubules, Proximal / chemistry*
  • Kidney Tubules, Proximal / pathology
  • Molecular Sequence Data
  • Neoplasm Proteins / analysis*
  • Peptide Mapping*

Substances

  • Neoplasm Proteins

Associated data

  • SWISSPROT/P00367
  • SWISSPROT/P00938
  • SWISSPROT/P02248
  • SWISSPROT/P02489
  • SWISSPROT/P02511
  • SWISSPROT/P02570
  • SWISSPROT/P02768
  • SWISSPROT/P04040
  • SWISSPROT/P04179
  • SWISSPROT/P05387
  • SWISSPROT/P05976
  • SWISSPROT/P06576
  • SWISSPROT/P07237
  • SWISSPROT/P09211
  • SWISSPROT/P09382
  • SWISSPROT/P10599
  • SWISSPROT/P10809
  • SWISSPROT/P16219
  • SWISSPROT/P19404
  • SWISSPROT/P20674
  • SWISSPROT/P24534
  • SWISSPROT/P27797
  • SWISSPROT/P30049
  • SWISSPROT/P30101
  • SWISSPROT/P38646
  • SWISSPROT/P47985
  • SWISSPROT/P51970