Triplex DNA: fundamentals, advances, and potential applications for gene therapy

J Mol Med (Berl). 1997 Apr;75(4):267-82. doi: 10.1007/s001090050112.


The ability to target specific sequences of DNA through oligonucleotide-based triple-helix formation provides a powerful tool for genetic manipulation. Under experimental conditions, triplex DNA can inhibit DNA transcription and replication, generate site-specific mutations, cleave DNA, and induce homologous recombination. This review describes the binding requirements for triplex formation, surveys recent advancements in the chemistry and biology of triple helices and considers several potential applications of triplex DNA for use in genetic therapy.

Publication types

  • Review

MeSH terms

  • Animals
  • DNA / therapeutic use*
  • Genetic Therapy / methods*
  • Humans
  • Nucleic Acid Conformation*
  • Oligodeoxyribonucleotides


  • Oligodeoxyribonucleotides
  • triplex DNA
  • DNA