Structural and serological characterisation of the O-antigenic polysaccharide of the lipopolysaccharide from Acinetobacter junii strain 65

Eur J Biochem. 1997 Apr 15;245(2):477-81. doi: 10.1111/j.1432-1033.1997.t01-1-00477.x.

Abstract

A polysaccharide containing rhamnose (Rha) and Gal was isolated by acetic acid hydrolysis, followed by gel-permeation chromatography, from the water-soluble lipopolysaccharide (phenol/water extracted) from Acinetobacter junii strain 65. The polysaccharide was characterised by means of monosaccharide analyses, Smith degradation, and NMR studies, and was shown to have a linear pentasaccharide repeating unit, as depicted below. This structure was specifically recognised in western blots and enzyme immunoassays by polyclonal rabbit antisera. [structure in text]

MeSH terms

  • Acinetobacter / chemistry*
  • Acinetobacter / classification
  • Acinetobacter / immunology
  • Animals
  • Blotting, Western
  • Carbohydrate Sequence
  • Electrophoresis, Polyacrylamide Gel
  • Galactose / analysis
  • Gas Chromatography-Mass Spectrometry
  • Immunoenzyme Techniques
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • O Antigens / chemistry*
  • O Antigens / immunology
  • Rabbits
  • Rhamnose / analysis

Substances

  • O Antigens
  • O-antigen, Actinetobacter junii strain 65
  • Rhamnose
  • Galactose