Characterisation of expression of mDMAHP, a homeodomain-encoding gene at the murine DM locus

Hum Mol Genet. 1997 May;6(5):651-7. doi: 10.1093/hmg/6.5.651.


We examined the expression of the murine homologue of myotonic dystrophy associated homeodomain protein (mDMAHP) using two different strategies. The first approach, RT-PCR, detected spliced transcripts in a wide range of embryonic and adult tissues, in a pattern overlapping substantially with the expression of mDMPK. A second approach, the generation of transgenic mice expressing the lacZ reporter gene from a 4.3 kb promoter fragment, also demonstrated expression in a range of tissues with potential links to the phenotype in myotonic dystrophy. We conclude that murine DMAHP has a similar pattern of expression to human DMAHP and will serve as a useful model for functional studies of this gene, although species differences, such as the reduced CpG island (1.8 kb compared with 3.5 kb) must be carefully considered.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CpG Islands
  • Embryo, Mammalian / metabolism
  • Female
  • Gene Expression Regulation, Developmental*
  • Genes, Reporter
  • Homeodomain Proteins / genetics*
  • Homeodomain Proteins / metabolism*
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Transgenic
  • Molecular Sequence Data
  • Myotonic Dystrophy / genetics*
  • Polymerase Chain Reaction
  • Pregnancy
  • RNA Splicing
  • RNA, Messenger / genetics
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Transcription, Genetic
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism


  • Homeodomain Proteins
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • SIX5 protein, human
  • Six5 protein, mouse
  • beta-Galactosidase