Transcriptional Analysis of the groESL Operon of Neisseria Gonorrhoeae

Gene. 1997 Apr 11;189(1):107-12. doi: 10.1016/s0378-1119(96)00842-6.

Abstract

The nucleotide sequence upstream of the groEL gene of Neisseria gonorrhoeae has been determined. Upstream of groEL is a homolog of groES and the divergently transcribed frpB gene. The promoter region of groES lacks the inverted repeat sequences (IR) that act as a regulatory element controlling the expression of similar operons in many other bacterial species. This region contains overlapping consensus sequences for sigma 32-dependent and sigma 70-dependent promoters, and an appropriately placed transcription start point was mapped downstream of these promoters. Northern hybridization demonstrated that synthesis of a full-length groES-groEL transcript was induced in heat-stressed cells. These experiments also revealed the presence of a shorter groES-specific transcript, apparently the result of the premature termination of transcription at an IR situated between the groES and groEL genes.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Chaperonins / genetics*
  • Cloning, Molecular
  • Escherichia coli Proteins
  • Genes, Bacterial*
  • Heat-Shock Proteins / genetics
  • Molecular Sequence Data
  • Neisseria gonorrhoeae / genetics*
  • Operon* / genetics
  • Sequence Analysis, DNA
  • Transcription, Genetic*

Substances

  • Bacterial Proteins
  • Escherichia coli Proteins
  • GroE protein, E coli
  • GroESL protein, Bacteria
  • Heat-Shock Proteins
  • Chaperonins

Associated data

  • GENBANK/U12277
  • GENBANK/U19726
  • GENBANK/U31917
  • GENBANK/U64996
  • GENBANK/U99443