Brain incorporation of [1-11C]arachidonate in normocapnic and hypercapnic monkeys, measured with positron emission tomography

Brain Res. 1997 Apr 25;755(1):74-83. doi: 10.1016/s0006-8993(97)00088-7.


Positron emission tomography (PET) was used to determine brain incorporation coefficients k* of [1-11C]arachidonate in isoflurane-anesthetized rhesus monkeys, as well as cerebral blood flow (CBF) using [15O]water. Intravenously injected [1-11C]arachidonate disappeared from plasma with a half-life of 1.1 min, whereas brain radioactivity reached a steady-state by 10 min. Mean values of k* were the same whether calculated by a single-time point method at 20 min after injection began, or by least-squares fitting of an equation for total brain radioactivity to data at all time points. k* equalled 1.1-1.2 x 10(-4) ml x s(-1) x g(-1) in gray matter and was unaffected by a 2.6-fold increase in CBF caused by hypercapnia. These results indicate that brain incorporation of [1-11C]arachidonate can be quantified in the primate using PET, and that incorporation is flow-independent.

MeSH terms

  • Animals
  • Arachidonic Acid / metabolism*
  • Brain / diagnostic imaging
  • Brain / metabolism*
  • Carbon Radioisotopes
  • Cerebrovascular Circulation / physiology*
  • Female
  • Hypercapnia / metabolism*
  • Least-Squares Analysis
  • Macaca mulatta
  • Male
  • Phospholipids / metabolism*
  • Radioligand Assay
  • Reference Values
  • Tomography, Emission-Computed


  • Carbon Radioisotopes
  • Phospholipids
  • Arachidonic Acid