Proton magnetic resonance spectroscopy of the brain during acute hypoxia-ischemia and delayed cerebral energy failure in the newborn piglet

Pediatr Res. 1997 Jun;41(6):795-802. doi: 10.1203/00006450-199706000-00001.


Studies of the brains of severely birth-asphyxiated infants using proton (1H) magnetic resonance spectroscopy (MRS) have shown changes indicating a rise in cerebral lactate (Lac) and a fall in N-acetylaspartate (Naa). The aim of this study was to test two hypotheses: 1) that these changes can be reproduced in the newborn piglet after transient reversed cerebral hypoxiaischemia, and their time course determined; and 2) that changes in Lac peak-area ratios are related to changes in phosphorylation potential as determined by phosphorus (31P) MRS. Eighteen piglets aged < 24 h were anesthetized and ventilated. Twelve underwent temporary occlusion of the carotid arteries and hypoxemia, and six served as sham-operated controls. 1H and 31P spectra were acquired alternately, both during the insult and for the next 48 h, using a 7-tesla spectrometer. During hypoxiaischemia, the median Lac/total creatine (Cr) peak-area ratio rose from a baseline of 0.14 (interquartile range 0.07-0.27), to a maximum of 4.34 (3.33-7.45). After resuscitation, Lac/Cr fell to 0.75 (0.45-1.64) by 2 h, and then increased again to 2.43 (1.13-3.08) by 48 h. At all stages after resuscitation Lac/Cr remained significantly above baseline and control values. Naa/Cr was significantly reduced below baseline and control values by 48 h after resuscitation. The increases in the Lac peak-area ratios were concomitant with the falls in the [phosphocreatine (PCr)*]/ [inorganic phosphate (Pi)] ratio, during both acute hypoxiaischemia and delayed energy failure. The maximum Lac/Naa during delayed energy failure correlated strongly with the minimum [nucleotide triphosphate (NTP)]/[exchangeable phosphate pool (EPP)] (r = -0.94, p < 0.0001). We conclude that both hypotheses have been confirmed.

MeSH terms

  • Animals
  • Animals, Newborn
  • Aspartic Acid / analogs & derivatives
  • Aspartic Acid / metabolism
  • Asphyxia Neonatorum
  • Brain / metabolism*
  • Creatine / metabolism
  • Energy Metabolism*
  • Humans
  • Hydrogen
  • Hypoxia, Brain / metabolism*
  • Infant, Newborn
  • Ischemic Attack, Transient / metabolism*
  • Kinetics
  • Lactates / metabolism
  • Magnetic Resonance Spectroscopy / methods
  • Phosphocreatine / metabolism
  • Phosphorus
  • Reperfusion
  • Swine
  • Time Factors


  • Lactates
  • Phosphocreatine
  • Phosphorus
  • Aspartic Acid
  • Hydrogen
  • N-acetylaspartate
  • Creatine