Cloning and Sequence Analysis of zooA, a Streptococcus Zooepidemicus Gene Encoding a Bacteriocin-Like Inhibitory Substance Having a Domain Structure Similar to That of Lysostaphin

Gene. 1997 Apr 21;189(2):255-61. doi: 10.1016/s0378-1119(96)00859-1.


The nucleotide sequence has been determined for zooA, a gene encoding the bacteriocin-like inhibitory substance zoocin A in Streptococcus zooepidemicus strain 4881. The zooA gene product corresponds to the 285-amino acid (aa) zoocin A pre-peptide from which a leader sequence is cleaved to form the 262-aa biologically active molecule of estimated molecular mass 27,877 Da. Expression of zooA in a Gram-negative host was shown by the extracellular release from Escherichia coli, containing cloned zooA, of a biologically active peptide having an identical range of anti-bacterial activity to that of zoocin A, purified from S. zooepidemicus strain 4881. Data base searches revealed sequences having homologies with known muralytic proteins produced by both Gram-positive and Gram-negative bacteria and indicate a 'mix and match' blending of domain-type structures, the C-terminal putative receptor-recognition region of the molecule being joined by a threonine-proline-rich linker to an N-terminal putative catalytic region having homology with several known endopeptidases, including lysostaphin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / isolation & purification*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / isolation & purification*
  • Bacteriocins / chemistry*
  • Base Sequence
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Genes, Bacterial*
  • Lysostaphin / chemistry*
  • Molecular Sequence Data
  • Peptides*
  • Protein Structure, Tertiary
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Streptococcus equi / chemistry
  • Streptococcus equi / enzymology
  • Streptococcus equi / genetics*


  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Bacteriocins
  • Peptides
  • ZooA protein, Streptococcus equi
  • Lysostaphin

Associated data

  • GENBANK/U50357