Brucella Abortus Strain 2308 Putative Glucose and Galactose Transporter Gene: Cloning and Characterization

Microbiology. 1997 May;143 ( Pt 5):1549-55. doi: 10.1099/00221287-143-5-1549.

Abstract

The gene for the putative transporter for glucose and galactose from Brucella abortus strain 2308 was isolated by functional complementation of Escherichia coli strains lacking either glucose or galactose transport systems. The same two plasmid clones were isolated from each screen. These clones restored glucose and galactose transport to the respective E. coli strains. The sequence of the 1806 bp overlap between these two plasmids was determined. A 1242 bp ORF whose disruption eliminated complementation of both E. coli strains showed 36% identity with the E. coli fucP gene encoding a fucose transporter. These two transporters are members of the major facilitator superfamily, in which they represent a previously undescribed family. In addition, an incomplete gene similar to E. coli hisG was found. One of the plasmids complemented E. coli hisG mutants.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Brucella abortus / genetics*
  • Brucella abortus / metabolism
  • Carrier Proteins / genetics
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Escherichia coli Proteins*
  • Galactose / metabolism*
  • Genes, Bacterial*
  • Genetic Complementation Test
  • Glucose / metabolism*
  • Molecular Sequence Data
  • Monosaccharide Transport Proteins / genetics*
  • Multigene Family*
  • Phylogeny
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Symporters*

Substances

  • Bacterial Proteins
  • Carrier Proteins
  • Escherichia coli Proteins
  • FucP protein, E coli
  • HisG protein, bacteria
  • Monosaccharide Transport Proteins
  • Symporters
  • Glucose
  • Galactose

Associated data

  • GENBANK/U43785
  • SWISSPROT/P11551