Abstract
The interaction of a bioengineered serpin (LEX032) with human leukocyte proteinase 3 (PR 3) has been investigated. LEX032 was found to be a time-dependent inhibitor of PR 3, forming a highly-stable enzyme-inhibitor complex (Ki 12 nM).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Binding Sites / genetics
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Humans
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In Vitro Techniques
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Kinetics
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Leukocytes / enzymology*
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Myeloblastin
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Recombinant Proteins / genetics
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Recombinant Proteins / pharmacology
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Serine Endopeptidases / metabolism*
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Serine Proteinase Inhibitors / genetics
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Serine Proteinase Inhibitors / pharmacology*
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Serpins / genetics
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Serpins / pharmacology*
Substances
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LEX 032
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Recombinant Proteins
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Serine Proteinase Inhibitors
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Serpins
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Serine Endopeptidases
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Myeloblastin