Molecular cloning of the interleukin-1 gene cluster: construction of an integrated YAC/PAC contig and a partial transcriptional map in the region of chromosome 2q13

Genomics. 1997 May 1;41(3):370-8. doi: 10.1006/geno.1997.4654.


Genes of the interleukin-1 (IL-1) gene cluster localized on chromosome 2q13 are implicated in many physiological and pathophysiological processes. We present here a high-resolution physical map of this region between markers D2S2008 and D2S4/PAX8. An integrated YAC/PAC contig and a partial transcriptional map were constructed by STS-constent mapping using the CEPH YAC library and three PAC libraries. A total of 3 YACs, 34 PACs, and 56 STSs were integrated: 33 newly generated probes to PAC end sequences, 9 polymorphic and 4 nonpolymorphic markers, 5 known genes, 4 expressed sequence tags, and 1 pseudogene. Within the map, a complete PAC contig of > 1 Mb encompasses the IL-1 gene cluster and PAX8, a paired-box-containing gene. This allowed us to define the transcriptional orientation of GLVR1, IL1B, and IL1RN and to show that PAX8 is localized outside the IL-1 gene cluster. FISH analysis localized PAC clones containing the IL-1 gene cluster to 2q12-q13. The data provide the basis for further characterization of the IL-1 gene cluster and for the construction of a sequence-ready PAC contig of this region.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Artificial, Yeast / genetics
  • Chromosomes, Human, Pair 2 / genetics*
  • Cloning, Molecular
  • DNA Primers / genetics
  • Gene Expression
  • Genetic Markers
  • Humans
  • In Situ Hybridization, Fluorescence
  • Interleukin-1 / genetics*
  • Molecular Sequence Data
  • Multigene Family*
  • Sequence Tagged Sites


  • DNA Primers
  • Genetic Markers
  • Interleukin-1

Associated data

  • GENBANK/R26859
  • GENBANK/U68351
  • GENBANK/U68352
  • GENBANK/U68353
  • GENBANK/U68354
  • GENBANK/U68355
  • GENBANK/U68356
  • GENBANK/U68357
  • GENBANK/U68358
  • GENBANK/U68359
  • GENBANK/U68360
  • GENBANK/U68361
  • GENBANK/U68362
  • GENBANK/U68363
  • GENBANK/U68364
  • GENBANK/U68365
  • GENBANK/U68366
  • GENBANK/U68367
  • GENBANK/U68368
  • GENBANK/U68369
  • GENBANK/U68370
  • GENBANK/U68371
  • GENBANK/U68372
  • GENBANK/U68373
  • GENBANK/U68374
  • GENBANK/U68375
  • GENBANK/U68376
  • GENBANK/U68377
  • GENBANK/U68378
  • GENBANK/U68379