The resistance and integrase genes of pACM1, a conjugative multiple-resistance plasmid, from Klebsiella oxytoca

Plasmid. 1997;37(2):105-18. doi: 10.1006/plas.1997.1284.


pACM1 is an 85-kb conjugative plasmid from a clinical isolate of Klebsiella oxytoca that encodes resistance to beta-lactams (mediated by SHV-5 extended spectrum beta-lactamase), trimethoprim, sulfonamides, tetracycline, aminoglycosides, and mercuric chloride. The expression of the aminoglycoside resistance is difficult to detect, which could have clinical implications. A region of pACM1 containing five resistance genes and two putative integrons was characterized by restriction mapping and partial DNA sequencing. One integron appears to be class I (sull type); the second lacks a recognizable 3' conserved segment. Neither integron has the BamHI site predicted for the 5' conserved segment. Plasmids encoding SHV-5 from other bacterial strains appear to be closely related to pACM1 by restriction enzyme analysis, but have resistance/ integron regions that vary in size and content from that of pACM1. Integrase-mediated recombination might be responsible for genetic divergence in a widely distributed family of pACM1-like plasmids.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Conjugation, Genetic
  • DNA, Bacterial / genetics
  • Drug Resistance, Multiple / genetics
  • Genes, Bacterial*
  • Genes, MDR*
  • Humans
  • Integrases / genetics*
  • Klebsiella / enzymology*
  • Klebsiella / genetics*
  • Klebsiella / isolation & purification
  • Molecular Sequence Data
  • R Factors / genetics*
  • Restriction Mapping


  • DNA, Bacterial
  • Integrases

Associated data

  • GENBANK/AJ009820
  • GENBANK/U90945