Five Complete Genomes of JC Virus Type 3 From Africans and African Americans

Arch Virol. 1997;142(4):637-55. doi: 10.1007/s007050050108.


The central demyelinating disease progressive multifocal leukoencephalopathy (PML) is caused by the human polyomavirus JC virus (JCV). JCV evolved as geographically based genotypes of which Type 3 is an African variant first characterized in HIV-1 positive patients from Tanzania. This study reports the complete sequence of five JCV Type 3 strains. The entire JCV genome was PCR amplified from urine specimens of three African and two African-American individuals. The African consensus sequence was compared to the Type 1 and Type 2 prototype strains, JCV (Mad-1) and JCV(GS/B), respectively. Type 3 differed in 2.2% of its coding region genome from JCV (Mad-1) and in 1.3% from JCV(GS/B). Within the coding region the sequence variation among the three types was higher in the capsid protein VP1 and in the regulatory protein large T antigen than in the agnoprotein or in VP2/3. Notable Type 3-specific changes were located at sites adjacent to the zinc finger motif and near the major donor and acceptor splice junctions of large T antigen. Four of the five urinary Type 3 strains had an unrearranged, archetypal regulatory region. African strain #309 showed a 10-bp deletion at a location similar to that previously described for #307 from Tanzania. The African-American Type 3 strain #312 was closely related to the African consensus sequence. The complete genome of a urinary JCV strain from another African-American male, previously reported as a possible Type 5, showed a sequence difference of only 0.52% from the Tanzanian consensus and has been reclassified as a subtype of Type 3.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • African Continental Ancestry Group*
  • Amino Acid Sequence
  • Base Sequence
  • DNA, Viral
  • Genome, Viral*
  • Humans
  • JC Virus / genetics*
  • JC Virus / isolation & purification
  • Male
  • Molecular Sequence Data
  • Regulatory Sequences, Nucleic Acid
  • Replication Origin / genetics
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Species Specificity
  • Tanzania
  • United States
  • Viral Proteins / chemistry
  • Viral Proteins / genetics


  • DNA, Viral
  • Viral Proteins

Associated data

  • GENBANK/D13942
  • GENBANK/J02227
  • GENBANK/J02288
  • GENBANK/J02400
  • GENBANK/K02449
  • GENBANK/M20322
  • GENBANK/M20775
  • GENBANK/M35834
  • GENBANK/M57473
  • GENBANK/U21836
  • GENBANK/U21837
  • GENBANK/U21838
  • GENBANK/U21839
  • GENBANK/U21840
  • GENBANK/U21841
  • GENBANK/U21842
  • GENBANK/U21843
  • GENBANK/U21844
  • GENBANK/U73178
  • GENBANK/U73500
  • GENBANK/V01108
  • GENBANK/X02449