Isolation of a bacterium that reductively dechlorinates tetrachloroethene to ethene

Science. 1997 Jun 6;276(5318):1568-71. doi: 10.1126/science.276.5318.1568.


Tetrachloroethene is a prominent groundwater pollutant that can be reductively dechlorinated by mixed anaerobic microbial populations to the nontoxic product ethene. Strain 195, a coccoid bacterium that dechlorinates tetrachloroethene to ethene, was isolated and characterized. Growth of strain 195 with H2 and tetrachloroethene as the electron donor and acceptor pair required extracts from mixed microbial cultures. Growth of strain 195 was resistant to ampicillin and vancomycin; its cell wall did not react with a peptidoglycan-specific lectin and its ultrastructure resembled S-layers of Archaea. Analysis of the 16S ribosomal DNA sequence of strain 195 indicated that it is a eubacterium without close affiliation to any known groups.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biodegradation, Environmental
  • Carcinogens / metabolism*
  • Culture Media
  • Ethylenes / metabolism*
  • Gram-Negative Bacteria / classification
  • Gram-Negative Bacteria / isolation & purification
  • Gram-Negative Bacteria / metabolism*
  • Gram-Negative Bacteria / ultrastructure
  • Phylogeny
  • RNA, Ribosomal, 16S / classification
  • Tetrachloroethylene / metabolism*
  • Trichloroethylene / metabolism
  • Water Pollutants, Chemical / metabolism


  • Carcinogens
  • Culture Media
  • Ethylenes
  • RNA, Ribosomal, 16S
  • Water Pollutants, Chemical
  • Trichloroethylene
  • ethylene
  • Tetrachloroethylene

Associated data

  • GENBANK/AF004928