Cloning, characterization, and sequence analysis of the clcE gene encoding the maleylacetate reductase of Pseudomonas sp. strain B13

J Bacteriol. 1997 Jun;179(11):3801-3. doi: 10.1128/jb.179.11.3801-3803.1997.

Abstract

A 3,167-bp PstI fragment of genomic DNA from Pseudomonas sp. strain B13 was cloned and sequenced. The gene clcE consists of 1,059 nucleotides encoding a protein of 352 amino acids with a calculated mass of 37,769 Da which showed maleylacetate reductase activity. The protein had significant sequence similarities with the polypeptides encoded by tcbF of pP51 (59.4% identical positions), tfdF of pJP4 (55.1%), and tftE of Burkholderia cepacia AC1100 (53.1%). The function of TcbF as maleylacetate reductase was established by an enzyme assay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Oxidoreductases / genetics*
  • Oxidoreductases Acting on CH-CH Group Donors*
  • Pseudomonas / enzymology
  • Pseudomonas / genetics*
  • Sequence Alignment
  • Sequence Analysis

Substances

  • Oxidoreductases
  • Oxidoreductases Acting on CH-CH Group Donors
  • maleylacetate reductase

Associated data

  • GENBANK/AF019038