A Functional Neo-Centromere Formed Through Activation of a Latent Human Centromere and Consisting of Non-Alpha-Satellite DNA

Nat Genet. 1997 Jun;16(2):144-53. doi: 10.1038/ng0697-144.

Abstract

We recently described a human marker chromosome containing a functional neo-centromere that binds anti-centromere antibodies, but is devoid of centromeric alpha-satellite repeats and derived from a hitherto non-centromeric region of chromosome 10q25. Chromosome walking using cloned single-copy DNA from this region enabled us to identify the antibody-binding domain of this centromere. Extensive restriction mapping indicates that this domain has an identical genomic organization to the corresponding normal chromosomal region, suggesting a mechanism for the origin of this centromere through the activation of a latent centromere that exists within 10q25.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites, Antibody
  • Centromere*
  • Chromosomes, Artificial, Yeast
  • Chromosomes, Human, Pair 10*
  • DNA, Satellite / genetics*
  • DNA, Satellite / metabolism
  • Genetic Markers
  • Humans
  • Hybrid Cells
  • In Situ Hybridization, Fluorescence

Substances

  • DNA, Satellite
  • Genetic Markers