Presenilin 1 interaction in the brain with a novel member of the Armadillo family

Neuroreport. 1997 Apr 14;8(6):1489-94. doi: 10.1097/00001756-199704140-00033.


One approach to understanding the function of presenilin 1 (PS1), is to discover those proteins with which it interacts. Evidence for a function in developmental patterning came from C. elegans, in which a PS homologue was identified by screening for suppressors of a mutation in Notch/lin-12, a gene which specifies cell fate. However, this genetic experiment cannot determine which proteins directly interact with PS1. Therefore, we utilized the two hybrid system and confirmatory co-immunoprecipitations to identify a novel catenin, termed gamma-catenin, which interacts with PS1 and is principally expressed in brain. The catenins are a gene family related to the Armadillo gene in Drosophila, some of which appear to have dual roles-they are components of cell-cell adherens junctions, and may serve as intermediates in the Wingless (Wg) signaling pathway, which, like Notch/lin-12, is also responsible for a variety of inductive signaling events. In the non-neuronal 293 cell line, PS1 interacted with gamma-catenin, the family member with the greatest homology to Armadillo. Wg and Notch interactions are mediated by the Disheveled gene, which may form a signaling complex with PS1 and Wg pathway intermediates to regulate the function of the Notch/lin-12 gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / metabolism*
  • Cytoskeletal Proteins / genetics*
  • Desmoplakins
  • Drosophila / genetics*
  • Gene Expression Regulation, Developmental / physiology*
  • Genes, Insect*
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Presenilin-1
  • Sequence Homology, Nucleic Acid
  • Trans-Activators*
  • beta Catenin
  • gamma Catenin


  • Cytoskeletal Proteins
  • Desmoplakins
  • Membrane Proteins
  • Presenilin-1
  • Trans-Activators
  • beta Catenin
  • gamma Catenin

Associated data

  • GENBANK/X79293
  • GENBANK/X81889
  • GENBANK/Z17804