Brucella abortus siderophore 2,3-dihydroxybenzoic acid protects brucellae from killing by macrophages

Vet Res. 1997;28(1):87-92.

Abstract

Addition of 2,3-dihydroxybenzoic acid, a siderophore produced by Brucella abortus, to macrophage cultures prevented intracellular killing of brucellae during the first 12 h after infection and increased the number of intracellular brucellae recovered at 48 h after infection. The protective effect could be demonstrated with inflammatory macrophages, interferon-gamma-activated macrophages and with macrophages supplemented with iron, shown elsewhere to facilitate killing of B abortus.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Binding, Competitive
  • Brucella abortus / drug effects
  • Brucella abortus / immunology*
  • Brucella abortus / metabolism
  • Cells, Cultured
  • Deferoxamine / metabolism
  • Deferoxamine / pharmacology
  • Dose-Response Relationship, Drug
  • Hydroxybenzoates / metabolism
  • Hydroxybenzoates / pharmacology*
  • Iron / metabolism
  • Macrophages, Peritoneal / drug effects
  • Macrophages, Peritoneal / immunology*
  • Macrophages, Peritoneal / microbiology
  • Mice
  • Mice, Inbred BALB C
  • Respiratory Burst
  • Siderophores / metabolism
  • Siderophores / pharmacology*

Substances

  • Hydroxybenzoates
  • Siderophores
  • 2,3-dihydroxybenzoic acid
  • Iron
  • Deferoxamine