Abstract
Addition of 2,3-dihydroxybenzoic acid, a siderophore produced by Brucella abortus, to macrophage cultures prevented intracellular killing of brucellae during the first 12 h after infection and increased the number of intracellular brucellae recovered at 48 h after infection. The protective effect could be demonstrated with inflammatory macrophages, interferon-gamma-activated macrophages and with macrophages supplemented with iron, shown elsewhere to facilitate killing of B abortus.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Binding, Competitive
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Brucella abortus / drug effects
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Brucella abortus / immunology*
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Brucella abortus / metabolism
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Cells, Cultured
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Deferoxamine / metabolism
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Deferoxamine / pharmacology
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Dose-Response Relationship, Drug
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Hydroxybenzoates / metabolism
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Hydroxybenzoates / pharmacology*
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Iron / metabolism
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Macrophages, Peritoneal / drug effects
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Macrophages, Peritoneal / immunology*
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Macrophages, Peritoneal / microbiology
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Mice
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Mice, Inbred BALB C
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Respiratory Burst
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Siderophores / metabolism
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Siderophores / pharmacology*
Substances
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Hydroxybenzoates
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Siderophores
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2,3-dihydroxybenzoic acid
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Iron
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Deferoxamine