Simultaneous determination of cytotoxic (adriamycin, vincristine) and modulator of resistance (verapamil, S 9788) drugs in human cells by high-performance liquid chromatography and ultraviolet detection

J Chromatogr B Biomed Sci Appl. 1997 Apr 11;691(2):449-56. doi: 10.1016/s0378-4347(96)00342-8.


Multidrug resistance (MDR), which was described for structurally and mechanistically unrelated anticancer agents, was modulated in vitro by a series of compounds which were of different chemical origin. In this situation, the selection of a correct assay dosage to study the MDR modulation mechanism was a problem. We developed a high-performance liquid chomatography (HPLC) method which enabled the simultaneous determination of three major cytotoxins (adriamycin, daunorubicin, vincristine) and two well-known modulators (S 9788, verapamil). This assay was fully validated and was used to follow, for the first time, the uptake and accumulation behaviour of adriamycin and S 9788 co-incubated with resistant and sensitive cell lines (KB-3-1; KB-A1).

MeSH terms

  • Antibiotics, Antineoplastic / analysis*
  • Antibiotics, Antineoplastic / pharmacology
  • Antineoplastic Agents / analysis*
  • Antineoplastic Agents / pharmacology
  • Chromatography, High Pressure Liquid
  • Doxorubicin / analysis
  • Doxorubicin / pharmacology
  • Drug Interactions
  • Drug Resistance, Multiple
  • Drug Resistance, Neoplasm
  • Humans
  • KB Cells
  • Piperidines / analysis
  • Piperidines / pharmacology
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrophotometry, Ultraviolet
  • Triazines / analysis
  • Triazines / pharmacology
  • Verapamil / analysis
  • Verapamil / pharmacology
  • Vincristine / analysis


  • Antibiotics, Antineoplastic
  • Antineoplastic Agents
  • Piperidines
  • Triazines
  • S 9788
  • Vincristine
  • Doxorubicin
  • Verapamil