NGF and BDNF increase the immunoreactivity of vesicular acetylcholine transporter in cultured neurons from the embryonic rat septum

Neurosci Lett. 1997 May 2;226(3):207-9. doi: 10.1016/s0304-3940(97)00284-x.

Abstract

The expression of vesicular acetylcholine transporter (VAChT), which transports ACh into synaptic vesicles, is coregulated with choline acetyltransferase (ChAT). Therefore, the effects of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) on the levels of VAChT in cultured neurons from the septum of embryonic rats were investigated by immunocytochemistry. NGF and BDNF increased the number of VAChT-immunoreactive neurons by approximately 1.5-fold and enhanced the immunoreactivity in each positive cell. These results suggest that the neurotrophins enhance not only synthesis but also storage of ACh in septal neurons.

MeSH terms

  • Acetylcholine*
  • Animals
  • Brain-Derived Neurotrophic Factor / pharmacology*
  • Carrier Proteins / analysis*
  • Cells, Cultured
  • Drug Evaluation, Preclinical
  • Immunohistochemistry
  • Membrane Transport Proteins*
  • Nerve Growth Factors / pharmacology*
  • Neurons / drug effects*
  • Rats
  • Septum Pellucidum / cytology
  • Septum Pellucidum / drug effects*
  • Septum Pellucidum / embryology
  • Vesicular Acetylcholine Transport Proteins
  • Vesicular Transport Proteins*

Substances

  • Brain-Derived Neurotrophic Factor
  • Carrier Proteins
  • Membrane Transport Proteins
  • Nerve Growth Factors
  • Slc18a3 protein, rat
  • Vesicular Acetylcholine Transport Proteins
  • Vesicular Transport Proteins
  • Acetylcholine