Diagnostic evaluation of PCR in goats experimentally infected with Trypanosoma vivax

Acta Trop. 1997 Jun 24;66(1):45-50. doi: 10.1016/s0001-706x(97)00677-3.


Six goats were experimentally infected with a stock of Trypanosoma vivax. Parasitaemia was weekly monitored by buffy coat and wet blood film examination during a period of 15 weeks and another 3 weeks following drug-treatment. Dried blood samples were tested by the polymerase chain reaction (PCR), using an extraction method with Chelex 100 (BioRad). PCR proved consistently more sensitive than the parasitological techniques.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Female
  • Goat Diseases / diagnosis*
  • Goat Diseases / parasitology
  • Goats / parasitology*
  • Polymerase Chain Reaction* / methods
  • Trypanosoma vivax
  • Trypanosomiasis, African / diagnosis
  • Trypanosomiasis, African / veterinary*