AtPLC2, a gene encoding phosphoinositide-specific phospholipase C, is constitutively expressed in vegetative and floral tissues in Arabidopsis thaliana

Plant Mol Biol. 1997 May;34(1):175-80. doi: 10.1023/a:1005885230896.


A cDNA encoding a phosphoinositide-specific phospholipase C (PI-PLC) from the higher plant Arabidopsis thaliana was cloned and characterized. The gene corresponding to this cDNA is designated AtPLC2. The overall structure of the predicted AtPLC2 protein is similar to those of plant PI-PLCs and mammalian delta-type PI-PLCs. Northern blot analysis revealed that AtPLC2 is expressed constitutively whereas AtPLC1S, another gene for PI-PLC of Arabidopsis, is induced by environmental stresses such as dehydration and salinity, indicating that the function of AtPLC2 is distinct from that of AtPLC1S. The AtPLC2 mRNA was detected in vegetative and floral tissues. We determined the positions of these two PI-PLCs genes on Arabidopsis chromosomes by RFLP mapping using P1 genomic clones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / enzymology*
  • Arabidopsis / genetics*
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / isolation & purification
  • Gene Expression Regulation, Plant*
  • Genes, Plant*
  • Molecular Sequence Data
  • Phosphatidylinositol Diacylglycerol-Lyase
  • Phosphoinositide Phospholipase C
  • Phosphoric Diester Hydrolases / biosynthesis
  • Phosphoric Diester Hydrolases / genetics*
  • Phosphoric Diester Hydrolases / isolation & purification
  • Plant Proteins / biosynthesis
  • Plant Proteins / genetics*
  • Plant Proteins / isolation & purification


  • DNA, Complementary
  • Plant Proteins
  • Phosphoric Diester Hydrolases
  • Phosphoinositide Phospholipase C
  • Phosphatidylinositol Diacylglycerol-Lyase

Associated data

  • GENBANK/D50804