Inhibition of growth and enhancement of differentiation of colorectal carcinoma cell lines by MAb MR6 and IL-4

Int J Cancer. 1997 May 16;71(4):605-11. doi: 10.1002/(sici)1097-0215(19970516)71:4<605::aid-ijc16>;2-a.


We have previously shown that expression of gp200-MR6, a molecule that is functionally associated with the interleukin-4 receptor (IL-4R), is lost from breast carcinoma cells as malignancy increases. Here we have analysed a series of colorectal carcinoma cell lines and show a similar decrease with increasing malignancy. Moreover, analysis of the HRA-19 cell line, which can exhibit a poorly or a well-differentiated phenotype according to culture conditions, shows that gp200-MR6 is weakly expressed on the former but strongly expressed on the latter. Functional analysis using either IL-4 or monoclonal antibody (MAb) MR6 and the well-differentiated cell line SW1222 revealed that MAb MR6 acts as an agonist for IL-4, with both reagents causing a dose-dependent inhibition of cell division, but greatly enhancing the glandular differentiation of SW1222 in three-dimensional collagen gels. These observations suggest that the gp200-MR6 molecule may act as the product of a tumour suppressor gene and that its loss may be a primary event in tumourigenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / pharmacology*
  • Antigens, CD*
  • Antigens, Neoplasm / immunology*
  • Carcinoma / pathology*
  • Cell Differentiation / drug effects
  • Cell Division / drug effects
  • Colorectal Neoplasms / pathology*
  • Depression, Chemical
  • Genes, Tumor Suppressor
  • Glycoproteins / immunology*
  • Glycoproteins / physiology
  • Humans
  • Interleukin-4 / pharmacology*
  • Lectins, C-Type*
  • Minor Histocompatibility Antigens
  • Receptors, Cell Surface*
  • Receptors, Interleukin*
  • Receptors, Interleukin-4
  • Tumor Cells, Cultured / drug effects


  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Neoplasm
  • DEC-205 receptor
  • Glycoproteins
  • Lectins, C-Type
  • Minor Histocompatibility Antigens
  • Receptors, Cell Surface
  • Receptors, Interleukin
  • Receptors, Interleukin-4
  • Interleukin-4