Prognostic significance of HER-2/neu gene amplification status by fluorescence in situ hybridization of prostate carcinoma

Cancer. 1997 Jun 1;79(11):2162-70. doi: 10.1002/(sici)1097-0142(19970601)79:11<2162::aid-cncr14>;2-u.


Background: HER-2/neu gene amplification, established as a prognostic factor in breast carcinoma and other cancers, has not been correlated with outcome in prostate carcinomas (PCs).

Methods: HER-2/neu gene amplification was determined by automated fluorescence in situ hybridization (FISH) using a unique sequence cosmid probe on 113 formalin fixed, paraffin embedded 4-microns tissue sections and the results compared with tumor grade, DNA ploidy, HER-2/neu protein expression by immunohistochemistry (IHC), serum prostate specific antigen, pathologic stage, and postoperative disease recurrence (mean follow-up of 44 months).

Results: HER-2/neu gene amplification by FISH (41% of PCs) correlated with tumor grade (P = 0.001) and DNA ploidy status (P = 0.0003). HER-2/neu protein overexpression by IHC (29% of PCs) correlated with grade (P = 0.03), but not with DNA ploidy. A trend for similar HER-2/neu status in each PC by IHC and FISH did not reach statistical significance (P = 0.25). On univariate analysis, HER-2/neu amplification by FISH (P = 0.029), tumor grade (P = 0.013), and DNA ploidy (P = 0.016) correlated with postoperative disease recurrence. HER-2/neu expression by IHC did not correlate with outcome. On multivariate analysis, grade (P = 0.0001) and ploidy (P = 0.001) were independent outcome predictors; HER-2/neu amplification by FISH reached near-independent significance (P = 0.125).

Conclusions: HER-2/neu gene amplification by FISH on archival PCs significantly correlates with grade and DNA ploidy status, is more sensitive than IHC in detecting HER-2/neu gene abnormalities, predicts postoperative disease recurrence, and may prove important in planning therapy for patients with prostate carcinoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / classification
  • Adenocarcinoma / pathology*
  • Aged
  • Aged, 80 and over
  • Breast Neoplasms / pathology
  • DNA, Neoplasm / analysis
  • Female
  • Gene Amplification*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Male
  • Middle Aged
  • Ploidies
  • Prognosis
  • Prostate-Specific Antigen / blood
  • Prostatectomy
  • Prostatic Neoplasms / classification
  • Prostatic Neoplasms / pathology*
  • Receptor, ErbB-2 / analysis*
  • Receptor, ErbB-2 / genetics


  • DNA, Neoplasm
  • Receptor, ErbB-2
  • Prostate-Specific Antigen