Molecular analysis of hus1+, a fission yeast gene required for S-M and DNA damage checkpoints

Mol Gen Genet. 1997 Apr 28;254(4):389-99. doi: 10.1007/pl00008606.


The structure of hus1+, a Schizosaccharomyces pombe gene required for S-M and DNA damage checkpoints, has been determined. Expression of hus1+ requires splicing of five exons, including a microexon that is only 13 nucleotides long. hus1+ is predicted to encode a 33 kDa protein with no similarity to sequences in any database, including the entire S. cerevisiae genome. Yeast strains disrupted for the hus1+ gene are viable but checkpoint-defective. Polyclonal antibodies were raised against bacterially expressed Hus1 protein, and used to study Hus1 regulation. Hus1 protein levels are not affected by S-phase arrest, and are not altered by mutations in other checkpoint genes, suggesting that Hus1 is not regulated at the transcriptional or translational levels.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Western
  • Cell Cycle Proteins / chemistry
  • Cell Cycle Proteins / genetics*
  • Cell Cycle Proteins / metabolism
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Damage / genetics*
  • DNA, Complementary
  • Exons / genetics
  • Gene Expression Regulation, Fungal / genetics
  • Genes, Fungal*
  • Introns / genetics
  • Microscopy, Fluorescence
  • Mitosis*
  • Molecular Sequence Data
  • Mutation / genetics
  • Open Reading Frames
  • S Phase*
  • Schizosaccharomyces / chemistry
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces pombe Proteins
  • Transcription, Genetic / genetics


  • Cell Cycle Proteins
  • DNA, Complementary
  • Schizosaccharomyces pombe Proteins
  • hus1 protein, S pombe

Associated data

  • GENBANK/Y09438