Enlarged peroxisomes are present in oleic acid-grown Yarrowia lipolytica overexpressing the PEX16 gene encoding an intraperoxisomal peripheral membrane peroxin

J Cell Biol. 1997 Jun 16;137(6):1265-78. doi: 10.1083/jcb.137.6.1265.


Pex mutants of the yeast Yarrowia lipolytica are defective in peroxisome assembly. The mutant strain pex16-1 lacks morphologically recognizable peroxisomes. Most peroxisomal proteins are mislocalized to a subcellular fraction enriched for cytosol in pex16 strains, but a subset of peroxisomal proteins is localized at, or near, wild-type levels to a fraction typically enriched for peroxisomes. The PEX16 gene was isolated by functional complementation of the pex16-1 strain and encodes a protein, Pex16p, of 391 amino acids (44,479 D). Pex16p has no known homologues. Pex16p is a peripheral protein located at the matrix face of the peroxisomal membrane. Substitution of the carboxylterminal tripeptide Ser-Thr-Leu, which is similar to the consensus sequence of peroxisomal targeting signal 1, does not affect targeting of Pex16p to peroxisomes. Pex16p is synthesized in wild-type cells grown in glucose-containing media, and its levels are modestly increased by growth of cells in oleic acid-containing medium. Overexpression of the PEX16 gene in oleic acid- grown Y. lipolytica leads to the appearance of a small number of enlarged peroxisomes, which contain the normal complement of peroxisomal proteins at levels approaching those of wild-type peroxisomes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carboxylic Acids
  • DNA, Fungal
  • Extracellular Matrix / metabolism
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Gene Expression
  • Genes, Fungal
  • Guinea Pigs
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Microbodies*
  • Molecular Sequence Data
  • Oleic Acid / pharmacology
  • Rabbits
  • Saccharomycetales / genetics*
  • Saccharomycetales / growth & development
  • Saccharomycetales / metabolism
  • Saccharomycetales / ultrastructure
  • Yeasts / genetics*
  • Yeasts / growth & development
  • Yeasts / metabolism
  • Yeasts / ultrastructure


  • Carboxylic Acids
  • DNA, Fungal
  • Fungal Proteins
  • Membrane Proteins
  • Oleic Acid