Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 89 (5), 755-64

Targeted Disruption of Cbfa1 Results in a Complete Lack of Bone Formation Owing to Maturational Arrest of Osteoblasts

Affiliations

Targeted Disruption of Cbfa1 Results in a Complete Lack of Bone Formation Owing to Maturational Arrest of Osteoblasts

T Komori et al. Cell.

Abstract

A transcription factor, Cbfa1, which belongs to the runt-domain gene family, is expressed restrictively in fetal development. To elucidate the function of Cbfa1, we generated mice with a mutated Cbfa1 locus. Mice with a homozygous mutation in Cbfa1 died just after birth without breathing. Examination of their skeletal systems showed a complete lack of ossification. Although immature osteoblasts, which expressed alkaline phophatase weakly but not Osteopontin and Osteocalcin, and a few immature osteoclasts appeared at the perichondrial region, neither vascular nor mesenchymal cell invasion was observed in the cartilage. Therefore, our data suggest that both intramembranous and endochondral ossification were completely blocked, owing to the maturational arrest of osteoblasts in the mutant mice, and demonstrate that Cbfa1 plays an essential role in osteogenesis.

Comment in

Similar articles

  • [CBFA1/PEBP2 Alpha A]
    T Komori. Nihon Rinsho 56 (6), 1430-4. PMID 9648460.
    A transcription factor, Cbfa1/Pebp2 alpha A, which belongs to runt-domain gene family, is preferentially expressed in the osteoblast lineage. Cbfa1/Pebp2 alpha A-deficien …
  • Maturational Disturbance of Chondrocytes in Cbfa1-deficient Mice
    M Inada et al. Dev Dyn 214 (4), 279-90. PMID 10213384.
    Cbfa1, a transcription factor that belongs to the runt-domain gene family, plays an essential role in osteogenesis. Cbfa1-deficient mice completely lacked both intramembr …
  • A Fundamental Transcription Factor for Bone and Cartilage
    T Komori. Biochem Biophys Res Commun 276 (3), 813-6. PMID 11027552. - Review
    Cbfa1-deficient mice were found to show a complete lack of bone formation owing to the maturational arrest of osteoblasts. Cbfa1 plays key roles in the determination of o …
  • Dentin Matrix Protein 1, a Target Molecule for Cbfa1 in Bone, Is a Unique Bone Marker Gene
    JQ Fen et al. J Bone Miner Res 17 (10), 1822-31. PMID 12369786.
    Dentin matrix protein 1 (Dmp1), a phosphoprotein highly linked to dentin formation, has also been reported to be expressed in the skeleton. However, the role of Dmp1 in s …
  • Cbfa1: A Molecular Switch in Osteoblast Biology
    P Ducy. Dev Dyn 219 (4), 461-71. PMID 11084646. - Review
    During the past 4 years, our molecular understanding of osteoblast biology has made rapid progress due to the characterization of the function of one molecule, Cbfa1. Thi …
See all similar articles

Cited by 1,103 PubMed Central articles

See all "Cited by" articles

Publication types

Substances

LinkOut - more resources

Feedback