Interleukin-17 up-regulation of nitric oxide production in human osteoarthritis cartilage

Arthritis Rheum. 1997 Jun;40(6):1050-3. doi: 10.1002/art.1780400609.


Objective: To examine the effect of human interleukin-17 (IL-17) on nitric oxide (NO) production in human osteoarthritis (OA) cartilage under ex vivo conditions.

Methods: OA cartilage from patients undergoing knee replacement surgery was used in explant assays to assess the effect of IL-17. NO production was measured by estimating the stable NO metabolite, nitrite, in conditioned medium.

Results: IL-17 augmented the spontaneous production of nitric oxide. This augmentation was sensitive to cycloheximide and pyrrolidine dithiocarbamate, but not to dexamethasone or soluble IL-1 receptor.

Conclusion: IL-17 augments nitric oxide production in OA cartilage via nuclear factor kappaB activation, but independently of IL-1beta signaling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Cartilage, Articular / metabolism*
  • Cytokines / physiology
  • Humans
  • Interleukin-17
  • Interleukins / physiology*
  • Knee Joint
  • Knee Prosthesis
  • Middle Aged
  • Nitric Oxide / biosynthesis*
  • Osteoarthritis / metabolism*
  • Up-Regulation


  • Cytokines
  • Interleukin-17
  • Interleukins
  • Nitric Oxide