Duplex PCR of the Y-27H39 and HPRT loci with reference to Japanese population data on the HPRT locus

Nihon Hoigaku Zasshi. 1997 Apr;51(2):67-9.


Using a duplex polymerase chain reaction (PCR) system, we successfully amplified GATA microsatellite loci Y-27H39 and HPRT on the Y and X chromosomes, respectively, and detected their alleles with a digoxigenin-labeled (GATA)6 probe. Among 450 unrelated Japanese including 225 females, we found 6 HPRT alleles ranging from 155 to 175 nucleotides with a simple repeat structure comprising 11 to 16 repeats. The duplex PCR is highly informative in Japanese.

MeSH terms

  • Alleles
  • Asian People
  • Female
  • Gene Frequency
  • Humans
  • Hypoxanthine Phosphoribosyltransferase / genetics*
  • Japan
  • Male
  • Microsatellite Repeats
  • Paternity
  • Polymerase Chain Reaction / methods
  • Polymorphism, Genetic
  • Sex Determination Analysis
  • X Chromosome
  • Y Chromosome*


  • Hypoxanthine Phosphoribosyltransferase