Duplex PCR of the Y-27H39 and HPRT loci with reference to Japanese population data on the HPRT locus

T Kishida; W Wang; M Fukuda; Y Tamaki

Duplex PCR of the Y-27H39 and HPRT loci with reference to Japanese population data on the HPRT locus

Nihon Hoigaku Zasshi. 1997 Apr;51(2):67-9.

Authors

T Kishida¹, W Wang, M Fukuda, Y Tamaki

Affiliation

¹ Department of Forensic Medicine, Oita Medical University, Japan.

PMID: 9184015

Abstract

Using a duplex polymerase chain reaction (PCR) system, we successfully amplified GATA microsatellite loci Y-27H39 and HPRT on the Y and X chromosomes, respectively, and detected their alleles with a digoxigenin-labeled (GATA)6 probe. Among 450 unrelated Japanese including 225 females, we found 6 HPRT alleles ranging from 155 to 175 nucleotides with a simple repeat structure comprising 11 to 16 repeats. The duplex PCR is highly informative in Japanese.

MeSH terms

Alleles
Asian People
Female
Gene Frequency
Humans
Hypoxanthine Phosphoribosyltransferase / genetics*
Japan
Male
Microsatellite Repeats
Paternity
Polymerase Chain Reaction / methods
Polymorphism, Genetic
Sex Determination Analysis
X Chromosome
Y Chromosome*

Substances

Hypoxanthine Phosphoribosyltransferase