A novel MAP-kinase kinase from Candida albicans

Gene. 1997 Apr 29;190(1):99-104. doi: 10.1016/s0378-1119(96)00758-5.

Abstract

A putative MAP-kinase kinase-encoding gene, CaSTE7, was isolated from Candida albicans by complementation of ste7 and ste11 mutants of the pheromone signal-transduction pathway of Saccharomyces cerevisiae. The nucleotide (nt) sequence revealed an ORF of 1767 nt encoding a putative protein of 589 amino acids (aa). CaSTE7 has a strong homology with MAP-kinase kinase STE7 of S. cerevisiae, the kinase domain having 45% homology with that of STE7. The deduced aa sequence contained all eleven consensus kinase subdomains found in MAP-kinase kinases. It can suppress the mating defect of ste5, ste11, ste7, and fus3 kss1 double mutants, but it cannot bypass the ste12 mutation. CaSTE7 behaves as a hyperactive allele of STE7, suppressing the mating defects of the pheromone signal-transduction pathway by constitutively stimulating STE12, and hence STE12-dependent processes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Candida albicans / enzymology*
  • DNA, Fungal
  • Fungal Proteins / metabolism
  • Mitogen-Activated Protein Kinase Kinases
  • Molecular Sequence Data
  • Protein Kinases / genetics
  • Protein Kinases / isolation & purification*
  • Protein Kinases / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Transcription Factors / metabolism

Substances

  • DNA, Fungal
  • Fungal Proteins
  • STE12 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Protein Kinases
  • Mitogen-Activated Protein Kinase Kinases

Associated data

  • GENBANK/U72980