Mitochondrial Acyl Carrier Protein Is Involved in Lipoic Acid Synthesis in Saccharomyces Cerevisiae

FEBS Lett. 1997 May 19;408(2):217-20. doi: 10.1016/s0014-5793(97)00428-6.

Abstract

The yeast gene, ACP1, encoding the mitochondrial acyl carrier protein, was deleted by gene replacement. The resulting acp1-deficient mutants had only 5-10% of the wild-type lipoic acid content remaining, and exhibited a respiratory-deficient phenotype. Upon meiosis, the lipoate deficiency co-segregated with the acp1 deletion. The role of ACP1 in long-chain fatty acid synthesis was studied in fast and fas2 null mutants completely lacking cytoplasmic fatty acid synthase. When grown on odd-chain (13:0 and 15:0) fatty acids, these cells showed less than 1% of C-16 and C-18 acids in their total lipids. Mitochondrial ACP is therefore suggested to be involved with the biosynthesis of octanoate, a precursor to lipoic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyl Carrier Protein / genetics
  • Acyl Carrier Protein / metabolism*
  • Cloning, Molecular
  • Fatty Acid Synthases / metabolism
  • Fatty Acids, Unsaturated / biosynthesis
  • Fatty Acids, Unsaturated / metabolism
  • Fungal Proteins / metabolism
  • Genes, Fungal
  • Meiosis
  • Mitochondria / enzymology
  • Mitochondria / metabolism*
  • Mutation
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Sequence Deletion
  • Thioctic Acid / analysis
  • Thioctic Acid / biosynthesis*

Substances

  • Acyl Carrier Protein
  • Fatty Acids, Unsaturated
  • Fungal Proteins
  • Thioctic Acid
  • Fatty Acid Synthases