High-performance liquid chromatographic determination of acrolein as a marker for cyclophosphamide bioactivation in human liver microsomes

J Chromatogr B Biomed Sci Appl. 1997 Apr 25;692(1):163-8. doi: 10.1016/s0378-4347(96)00457-4.


A high-performance liquid chromatographic method for the quantification of acrolein following incubation of cyclophosphamide (CP) with human liver microsomes was developed. Based on the formation of the fluorescent derivative 7-hydroxyquinoline by condensation of acrolein with 3-aminophenol quantitation was performed without prior extraction or other sample cleanup procedures. The method showed sufficient sensitivity with a limit of detection of 5 ng/ml and a limit of quantification of 10 ng/ml. The suitability of the method is shown for enzyme kinetic studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrolein / analysis*
  • Acrolein / pharmacokinetics
  • Aminophenols
  • Animals
  • Antineoplastic Agents, Alkylating / pharmacokinetics*
  • Biomarkers / analysis
  • Biotransformation
  • Chromatography, High Pressure Liquid
  • Cyclophosphamide / pharmacokinetics*
  • Humans
  • Microsomes, Liver / enzymology
  • Microsomes, Liver / metabolism*
  • Rats
  • Sensitivity and Specificity


  • Aminophenols
  • Antineoplastic Agents, Alkylating
  • Biomarkers
  • Acrolein
  • Cyclophosphamide
  • 3-aminophenol