Isolation of an anaerobic bacterium which reductively dechlorinates tetrachloroethene and trichloroethene

Biodegradation. 1996-1997;7(6):507-11. doi: 10.1007/BF00115297.

Abstract

Strain TEA, a strictly anaerobic, motile rod with one to four lateral flagella and a crystalline surface layer was isolated from a mixed culture that completely reduces chlorinated ethenes to ethene. The organism coupled reductive dehalogenation of tetrachloroethene or trichloroethene to cis-1,2-dichloroethene to growth, using molecular hydrogen as the electron donor. It was unable to grow fermentatively or in the presence of tri- or tetrachloroethene with glucose, pyruvate, lactate, acetate or formate. The 16S rDNA sequence of strain TEA was 99.7% identical to that of Dehalobacter restrictus. The two organisms thus are representatives of the same species or the same genus within the Bacillus/Clostridium subphylum of the gram-positive bacteria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria, Anaerobic / classification
  • Bacteria, Anaerobic / genetics
  • Bacteria, Anaerobic / growth & development
  • Bacteria, Anaerobic / metabolism*
  • Biodegradation, Environmental
  • Chlorides / metabolism
  • DNA, Bacterial / chemistry
  • DNA, Ribosomal / chemistry
  • Dichloroethylenes / metabolism
  • Electron Transport
  • Evolution, Molecular
  • Flagella / ultrastructure
  • Gram-Positive Bacteria / classification
  • Gram-Positive Bacteria / genetics
  • Gram-Positive Bacteria / growth & development
  • Gram-Positive Bacteria / metabolism
  • Hydrogen / metabolism
  • Microscopy, Electron
  • Molecular Sequence Data
  • Oxidation-Reduction
  • Oxidoreductases / metabolism
  • Polymerase Chain Reaction
  • Tetrachloroethylene / metabolism*
  • Trichloroethylene / metabolism*

Substances

  • Chlorides
  • DNA, Bacterial
  • DNA, Ribosomal
  • Dichloroethylenes
  • Trichloroethylene
  • Hydrogen
  • Oxidoreductases
  • Tetrachloroethylene
  • 1,2-dichloroethylene

Associated data

  • GENBANK/Y10164