Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 71 (7), 5481-6

Sendai Virus Efficiently Infects Cells via the Asialoglycoprotein Receptor and Requires the Presence of Cleaved F0 Precursor Proteins for This Alternative Route of Cell Entry

Affiliations

Sendai Virus Efficiently Infects Cells via the Asialoglycoprotein Receptor and Requires the Presence of Cleaved F0 Precursor Proteins for This Alternative Route of Cell Entry

M Bitzer et al. J Virol.

Abstract

Biochemical evidence suggests that the asialoglycoprotein receptor (ASGP-R) can be used as an alternative receptor for a temperature-sensitive Sendai virus (SV) mutant. We now have investigated this possible alternative route of infection for SV wild-type (SV-wt) strain Fushimi by using a pair of cell lines which differ only with regard to ASGP-R expression. Infection studies after enzymatic destruction of conventional sialic acid-containing SV receptors (SA-R) revealed that only ASGP-R-expressing cells could be infected by SV-wt. This alternative route of cell entry could be completely blocked by incubation of cells with ASGP-R-specific antibodies prior to infection. Furthermore, cleavage of SV-F0 precursor protein into the subunits F1 and F2 was necessary to establish infection via ASGP-R, suggesting a fusion-mediated cell entry after binding of SV-wt to the ASGP-R on host cells. Interestingly, infection via ASGP-R was found to be nearly as efficient as infection via conventional sialic acid-containing SV receptors. A possible physiological role of the ASGP-R-mediated route of SV infection is discussed.

Similar articles

See all similar articles

Cited by 10 PubMed Central articles

See all "Cited by" articles

References

    1. Int Rev Cytol. 1992;137B:181-219 - PubMed
    1. Targeted Diagn Ther. 1991;4:127-49 - PubMed
    1. Am J Respir Cell Mol Biol. 1993 Oct;9(4):361-70 - PubMed
    1. Biochim Biophys Acta. 1993 Oct 10;1152(1):15-25 - PubMed
    1. Virology. 1993 Nov;197(1):1-11 - PubMed

Publication types

LinkOut - more resources

Feedback