Cloning of a human homolog of the yeast OGG1 gene that is involved in the repair of oxidative DNA damage

Oncogene. 1997 Jun 12;14(23):2857-61. doi: 10.1038/sj.onc.1201139.


We report the cloning of a human homolog of the yeast OGGC1 gene, which encodes a DNA glycosylase that excises an oxidatively damaged form of guanine, 8-hydroxyguanine (also known as 7,8-dihydro-8-oxoguanine). Since the deduced amino acid sequence (68 amino acids) of a human expressed sequence tag, N55394, matched a short stretch of yeast OGG1 protein with greater than 40% amino acid identity, a full length cDNA clone was isolated from a HeLa cell cDNA library with the N55394 clone as a probe. The cDNA clone encodes a predicted protein of 345 amino acids which is homologous to yeast OGG1 protein throughout the entire polypeptide sequence and shares 38% amino acid identity with yeast OGG1 protein. Moreover, we found that both a human homolog and yeast OGG1 protein possess two distinct DNA binding motifs, a helix-hairpin-helix (HhH) motif and a C2H2 zinc finger like motif, and a domain homologous to human and E. coli MutY proteins. Expression of a human homolog suppressed spontaneous mutagenesis of an E. coli (mutM mutY) mutant as in the case of yeast OGG1 protein. The gene was ubiquitously expressed in a variety of human organs and mapped to chromosome 3p26.2. These results strongly suggest that the gene isolated here is a human counterpart of the yeast OGGI gene and is involved in the repair of oxidative DNA damage in human cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Damage*
  • DNA Repair / genetics*
  • DNA-Formamidopyrimidine Glycosylase
  • Humans
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • N-Glycosyl Hydrolases / genetics*
  • Oxidation-Reduction
  • Oxidative Stress
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid


  • N-Glycosyl Hydrolases
  • DNA-Formamidopyrimidine Glycosylase