Molecular cloning of a new unc-33-like cDNA from rat brain and its relation to paraneoplastic neurological syndromes

Brain Res Mol Brain Res. 1997 Jun;46(1-2):329-32. doi: 10.1016/s0169-328x(97)00080-6.


Anti-CV2-autoantibodies from patients with paraneoplastic neurological syndromes were used to purify protein(s) related to this disease. A novel cDNA, c-22, was obtained by PCR with primers based on amino-acid sequence of peptides obtained from this protein and rat brain cDNA as template. The deduced amino-acid sequence of c-22 shows homology to the Unc-33 gene from C. elegans in which mutations lead to defects in neuritic outgrowth and axonal guidance and cause uncoordinated movements of the nematode. Several consensus sites for putative protein kinase C phosphorylation were found, suggesting that the c-22 gene product may be a phosphoprotein. Northern hybridizations show that the apparently unique 3.8-kb mRNA of c-22 is present in rat brain tissue and its expression is developmentally regulated: the levels of C-22 mRNA, detectable in brain at embryonic day 17 (E17), increase up to post-natal day 7 (P7) and decline rapidly to an almost undetectable level in adult.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Brain / metabolism*
  • Brain Neoplasms / genetics*
  • Caenorhabditis elegans Proteins*
  • Cloning, Molecular*
  • DNA, Complementary / genetics
  • Helminth Proteins / genetics*
  • Humans
  • Molecular Sequence Data
  • Nerve Growth Factors / genetics*
  • Paraneoplastic Syndromes / genetics*
  • Rats


  • Caenorhabditis elegans Proteins
  • DNA, Complementary
  • Helminth Proteins
  • Nerve Growth Factors
  • unc-33 protein, C elegans

Associated data

  • GENBANK/U52095