Molecular cloning and characterization of a cDNA, CHEMR1, encoding a chemokine receptor with a homology to the human C-C chemokine receptor, CCR-4

Blood. 1997 Jun 15;89(12):4448-60.

Abstract

Chemokines refer to a rapidly expanding family of small cytokines whose primary function is recruitment of leukocytes to inflammatory sites. These are known to bind to seven-transmembrane-domain containing receptors. A cDNA clone, CHEMR1, resembling the typical G protein-coupled receptor, was isolated from a mouse cytotoxic T-lymphocyte (CTL) library. Northern blot analysis in mouse cell lines suggests that its expression is found in a variety of cells, including T cells, B cells, and macrophages. The CHEMR1 gene Scya3r2 is a single-copy gene whose open reading frame may be in a single exon and maps to the distal region of mouse Chr 9 where the mouse macrophage inflammatory protein-1alpha (MIP-1alpha) receptor gene Scya3r and two related C-C chemokine receptor-like genes reside. Amino acid sequence comparison shows that CHEMR1 is 84% identical to human CCR-4, indicating that CHEMR1 is likely to be a mouse CCR-4. Binding assays using 125I-labeled C-C chemokines in mammalian cells indicated that CHEMR1 did not bind MIP-1alpha, RANTES, or MIP-1beta, whereas CCR-1 binds MIP-1alpha and RANTES. Our result is different from the reported properties of human CCR-4. This suggests that CHEMR1 may be a receptor for unidentified C-C chemokine or a low-affinity receptor for MIP-1alpha.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chemokine CCL17
  • Chemokine CCL3
  • Chemokine CCL4
  • Chemokine CCL5 / metabolism
  • Chemokines / metabolism*
  • Chemokines, CC*
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Complementary / genetics*
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Gene Library
  • Genes*
  • Humans
  • Macrophage Inflammatory Proteins / metabolism
  • Mice / genetics*
  • Molecular Sequence Data
  • Organ Specificity
  • Receptors, CCR1
  • Receptors, Chemokine*
  • Receptors, Cytokine / metabolism
  • Recombinant Fusion Proteins / metabolism
  • Ribonucleases*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Substrate Specificity
  • T-Lymphocytes, Cytotoxic / chemistry*
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism

Substances

  • CCL17 protein, human
  • CCR1 protein, human
  • Ccl17 protein, mouse
  • Ccr1 protein, mouse
  • Chemokine CCL17
  • Chemokine CCL3
  • Chemokine CCL4
  • Chemokine CCL5
  • Chemokines
  • Chemokines, CC
  • DNA, Complementary
  • Fungal Proteins
  • Macrophage Inflammatory Proteins
  • Receptors, CCR1
  • Receptors, Chemokine
  • Receptors, Cytokine
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • CCR4 protein, S cerevisiae
  • Ribonucleases

Associated data

  • GENBANK/U15208