Overexpression of cyclin D1 correlates with early recurrence in superficial bladder cancers

Br J Cancer. 1997;75(12):1788-92. doi: 10.1038/bjc.1997.305.


Cyclin D1 is a cell cycle regulator essential for G1 phase progression and is frequently overexpressed in several human tumour types as a consequence of gene amplification or chromosomal rearrangements. We analysed the expression of cyclin D1 in 75 patients with transitional cell carcinoma (TCC) to investigate the possible relationship between its expression and clinical outcome as well as histopathological findings using the immunohistochemical method. We observed strong staining (++, > 50% positive cells) for cyclin D1 in 19 cases (25.3%) and weak staining (+, 5-50% positive cells) in 19 cases (25.3%). Overexpression of cyclin D1 was not associated with tumour invasion. No significant association was found between overexpression of cyclin D1 and tumour grade (P > 0.05). We assessed the differences of disease-free interval in superficial tumours and actuarial survival probability in invasive tumours according to the status of cyclin D1 expression. Tumours with (++) staining for cyclin D1 recurred much more rapidly than (-) and/or (+) staining tumours (P < 0.01 for - vs ++; P < 0.05 for + vs ++). However, overexpression of cyclin D1 was not associated with a shortened overall survival of patients with invasive tumours (P < 0.1). These results suggest that genetic alteration of cyclin D1 appears to be an early event in the tumorigenesis of bladder TCC and is associated with early recurrence in superficial tumours.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor*
  • Carcinoma, Transitional Cell / chemistry
  • Carcinoma, Transitional Cell / genetics*
  • Carcinoma, Transitional Cell / mortality
  • Cyclin D1
  • Cyclins / analysis
  • Cyclins / genetics*
  • Disease-Free Survival
  • Gene Expression
  • Humans
  • Immunohistochemistry
  • Middle Aged
  • Neoplasm Recurrence, Local
  • Oncogene Proteins / analysis
  • Oncogene Proteins / genetics*
  • Retrospective Studies
  • Time Factors
  • Urinary Bladder Neoplasms / chemistry
  • Urinary Bladder Neoplasms / genetics*
  • Urinary Bladder Neoplasms / mortality


  • Biomarkers, Tumor
  • Cyclins
  • Oncogene Proteins
  • Cyclin D1