Refined structures of three crystal forms of toxic shock syndrome toxin-1 and of a tetramutant with reduced activity

Protein Sci. 1997 Jun;6(6):1220-7. doi: 10.1002/pro.5560060610.

Abstract

The structure of toxic shock syndrome toxin-1 (TSST-1), the causative agent in toxic shock syndrome, has been determined in three crystal forms. The three structural models have been refined to R-factors of 0.154, 0.150, and 0.198 at resolutions of 2.05 A, 2.90 A, and 2.75 A, respectively. One crystal form of TSST-1 contains a zinc ion bound between two symmetry-related molecules. Although not required for biological activity, zinc dramatically potentiates the mitogenicity of TSST-1 at very low concentrations. In addition, the structure of the tetramutant TSST-1H [T69I, Y80W, E132K, I140T], which is nonmitogenic and does not amplify endotoxin shock, has been determined and refined in a fourth crystal form (R-factor = 0.173 to 1.9 A resolution).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Toxins / chemistry*
  • Bacterial Toxins / genetics
  • Bacterial Toxins / immunology
  • Crystallography, X-Ray
  • Enterotoxins / chemistry*
  • Enterotoxins / genetics
  • Enterotoxins / immunology
  • Mitogens / chemistry
  • Mitogens / genetics
  • Mitogens / immunology
  • Models, Molecular
  • Mutation
  • Protein Conformation
  • Staphylococcus aureus
  • Superantigens / chemistry*
  • Superantigens / genetics
  • Superantigens / immunology
  • Zinc / immunology

Substances

  • Bacterial Toxins
  • Enterotoxins
  • Mitogens
  • Superantigens
  • enterotoxin F, Staphylococcal
  • Zinc