Abstract
Connectin/titin, a 3000 kDa protein, links the Z line to the myosin filament in striated muscle sarcomeres. Using the yeast two-hybrid system, the present work shows that the N-terminal Z repeat 5 region (amino acids 447-472) of connectin binds to the C-terminal region (amino acids 825-897) of alpha-actinin, the main constituent of the Z line.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actinin / chemistry
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Actinin / metabolism*
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Amino Acid Sequence
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Animals
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Chickens
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Cloning, Molecular
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Connectin
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DNA, Complementary / genetics
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Humans
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Molecular Sequence Data
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Muscle Proteins / chemistry*
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Muscle Proteins / metabolism
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Muscle, Skeletal / chemistry
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Myocardium / chemistry
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Polymerase Chain Reaction
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Protein Binding
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Protein Kinases / chemistry*
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Protein Kinases / metabolism
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Saccharomyces cerevisiae
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Sarcomeres / chemistry
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Sequence Analysis
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Sequence Homology, Amino Acid
Substances
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Connectin
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DNA, Complementary
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Muscle Proteins
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TTN protein, human
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Actinin
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Protein Kinases