The N-terminal Z repeat 5 of connectin/titin binds to the C-terminal region of alpha-actinin

Biochem Biophys Res Commun. 1997 Jun 9;235(1):1-3. doi: 10.1006/bbrc.1997.6534.

Abstract

Connectin/titin, a 3000 kDa protein, links the Z line to the myosin filament in striated muscle sarcomeres. Using the yeast two-hybrid system, the present work shows that the N-terminal Z repeat 5 region (amino acids 447-472) of connectin binds to the C-terminal region (amino acids 825-897) of alpha-actinin, the main constituent of the Z line.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinin / chemistry
  • Actinin / metabolism*
  • Amino Acid Sequence
  • Animals
  • Chickens
  • Cloning, Molecular
  • Connectin
  • DNA, Complementary / genetics
  • Humans
  • Molecular Sequence Data
  • Muscle Proteins / chemistry*
  • Muscle Proteins / metabolism
  • Muscle, Skeletal / chemistry
  • Myocardium / chemistry
  • Polymerase Chain Reaction
  • Protein Binding
  • Protein Kinases / chemistry*
  • Protein Kinases / metabolism
  • Saccharomyces cerevisiae
  • Sarcomeres / chemistry
  • Sequence Analysis
  • Sequence Homology, Amino Acid

Substances

  • Connectin
  • DNA, Complementary
  • Muscle Proteins
  • TTN protein, human
  • Actinin
  • Protein Kinases

Associated data

  • GENBANK/AB001536