Identification and Characterization of a Novel monocyte/macrophage Differentiation-Dependent Gene That Is Responsive to Lipopolysaccharide, Ceramide, and Lysophosphatidylcholine

Biochem Biophys Res Commun. 1997 Jun 9;235(1):4-9. doi: 10.1006/bbrc.1997.6715.

Abstract

A novel differentiation-dependent cDNA (DIF-2) has been isolated from human mononuclear phagocytes by differential display. The full-length cDNA was cloned and sequenced. DIF-2 consists of 156 amino acids and has a predicted isoelectric point of 8.84. The mRNA is expressed in freshly isolated monocytes and is downregulated significantly when monocytes are subjected to differentiation. A similar differentiation-dependent downregulation is observed in normal hepatocytes compared to undifferentiated HepG2 cells. The mRNA expression in monocytes is sensitive to lipopolysaccharide and ceramide which both strongly increase DIF-2 transcription, while lysophosphatidylcholine results in a weaker upregulation of DIF-2 expression. A DIF-2 homologous gene has been previously isolated from mouse fibroblasts and was shown to be a serum growth factor-inducible immediate early gene. Our results indicate that DIF-2 represents a gene which is regulated in differentiation processes and strongly responsive to lipopolysaccharide, ceramide and lysophosphatidylcholine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Apoptosis Regulatory Proteins
  • Base Sequence
  • Cell Differentiation
  • Cells, Cultured
  • Ceramides / pharmacology*
  • Cloning, Molecular
  • Down-Regulation
  • Gene Expression Regulation / drug effects*
  • Glycoproteins / biosynthesis
  • Glycoproteins / chemistry
  • Glycoproteins / genetics*
  • Humans
  • Immediate-Early Proteins*
  • Isoelectric Point
  • Lipopolysaccharides / pharmacology*
  • Lysophosphatidylcholines / pharmacology*
  • Macrophages / cytology
  • Macrophages / metabolism
  • Membrane Proteins
  • Mice
  • Molecular Sequence Data
  • Monocytes / cytology
  • Monocytes / drug effects
  • Monocytes / metabolism*
  • Neoplasm Proteins*
  • Phosphoproteins / biosynthesis
  • Phosphoproteins / chemistry
  • Phosphoproteins / genetics*
  • RNA, Messenger / metabolism
  • Tetradecanoylphorbol Acetate / pharmacology
  • Tumor Cells, Cultured
  • Up-Regulation

Substances

  • Apoptosis Regulatory Proteins
  • Ceramides
  • Glycoproteins
  • IER3 protein, human
  • Immediate-Early Proteins
  • Lipopolysaccharides
  • Lysophosphatidylcholines
  • Membrane Proteins
  • Neoplasm Proteins
  • Phosphoproteins
  • RNA, Messenger
  • Tetradecanoylphorbol Acetate

Associated data

  • GENBANK/Y14551