TNF mediates lung leak, but not neutrophil accumulation, in lungs of rats given IL-1 intratracheally

Am J Respir Crit Care Med. 1997 Jun;155(6):1972-6. doi: 10.1164/ajrccm.155.6.9196104.


Interleukin-1 (IL-1) is increased in lung lavages obtained from patients with acute respiratory distress syndrome, and administering IL-1 intratracheally to rats causes an acute, neutrophil-dependent, oxidative lung leak. We found that rats given IL-1 intratracheally had increased lung lavage fluid tumor necrosis factor (TNF) levels, and that rats treated with TNF binding protein (TNFbp) intravenously did not develop the increased lung leak that occurs after administration of IL-1 intratracheally. In contrast, rats given IL-1 intratracheally and TNFbp intravenously had the same elevations in lung lavage neutrophil accumulation and lung lavage cytokine-induced neutrophil chemoattractant levels as rats given IL-1 intratracheally. Our results show that TNFbp decreases neutrophil-mediated lung leak, but not lung neutrophil accumulation, after administration of IL-1 intratracheally in rats.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Carrier Proteins / pharmacology
  • Humans
  • Indicators and Reagents / metabolism
  • Interleukin-1 / pharmacology*
  • Intubation, Intratracheal
  • Leukocyte Count / drug effects
  • Luminescent Measurements
  • Lung / drug effects*
  • Lung / metabolism*
  • Lung / pathology
  • Male
  • Neutrophils / pathology*
  • Nitroblue Tetrazolium / metabolism
  • Oxidation-Reduction / drug effects
  • Permeability
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Proteins
  • Therapeutic Irrigation
  • Tumor Necrosis Factor-alpha / metabolism
  • Tumor Necrosis Factor-alpha / physiology*


  • Carrier Proteins
  • Indicators and Reagents
  • Interleukin-1
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Nitroblue Tetrazolium