Human Supt5h protein, a putative modulator of chromatin structure, is reversibly phosphorylated in mitosis

FEBS Lett. 1997 Jun 2;409(1):74-8. doi: 10.1016/s0014-5793(97)00486-9.

Abstract

The Saccharomyces cerevisiae proteins Spt4p, Spt5p and Spt6p are involved in transcriptional repression by modulating the structure of chromatin. From HeLa cells we have purified a human homologue of Spt5p, Supt5hp, and show here that the protein is reversibly phosphorylated in mitosis. The cloned cDNA predicts a protein of 1087 residues with 31% identity to yeast Spt5p. It includes an acidic N-terminus, a putative nuclear localization signal and a C-terminal region containing two different repeated motifs. One of them, with the consensus sequence P-T/S-P-S-P-Q/A-S/G-Y, is similar to the C-terminal domain in the largest subunit of RNA polymerase II.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Cycle / physiology
  • Chromatin / chemistry*
  • Chromatin / drug effects
  • Chromatin / metabolism*
  • Chromosomal Proteins, Non-Histone / chemistry
  • Chromosomal Proteins, Non-Histone / genetics
  • Chromosomal Proteins, Non-Histone / metabolism*
  • Chromosomal Proteins, Non-Histone / pharmacology*
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / isolation & purification
  • HeLa Cells
  • Humans
  • Mitosis*
  • Molecular Sequence Data
  • Phosphorylation
  • Sequence Homology, Amino Acid
  • Transcription Factors / chemistry
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription Factors / pharmacology*
  • Transcriptional Elongation Factors*

Substances

  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • DNA, Complementary
  • Transcription Factors
  • Transcriptional Elongation Factors
  • SPT5 transcriptional elongation factor

Associated data

  • GENBANK/Y12790