Extensive maternal representation of DNA-binding proteins that interact with regulatory target sites of the Strongylocentrotus purpuratus CyIIIa gene

Mol Mar Biol Biotechnol. 1997 Jun;6(2):79-83.

Abstract

Nine different embryonic transcription factors interact at specific target sites in the cis-regulatory domain of the Strongylocentrotus purpuratus CyIIIa gene. We tested eight of these site sequences and show here that for every one a DNA-binding protein was present in unfertilized egg cytoplasm. The concentrations of active DNA-binding proteins per egg were estimated. We also present a new and convenient method for preparation of a material cytoplasmic fraction that retains these factors in active form.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / genetics*
  • Animals
  • Blastocyst / chemistry
  • Cytoplasm / chemistry
  • DNA-Binding Proteins / metabolism*
  • Female
  • Ovum / chemistry*
  • Protein Binding
  • Regulatory Sequences, Nucleic Acid*
  • Sea Urchins / embryology
  • Sea Urchins / genetics
  • Transcription Factors / metabolism*

Substances

  • Actins
  • DNA-Binding Proteins
  • Transcription Factors