In vitro and in vivo studies of murine sarcoma cells after prolonged treatment with promoting phorbol ester TPA

Neoplasma. 1997;44(1):25-9.

Abstract

Murine sarcoma cell line (L-1) treated with promoting phorbol ester (TPA) showed decreased content and activity of protein kinase C (PKC) as measured by Western blotting and histone phosphorylation methods. The PKC depleted line (L-1R) produced bigger, tumors after s.c. transplantation into syngeneic mice and more spontaneous and artificial metastases developing after i.v. injection of tumor cells. The in vitro studies revealed decreased: adhesiveness, migratory and invasiveness properties of PKC depleted cells. Negative correlation between in vitro and in vivo studies were found.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Carcinogens / pharmacology*
  • Cell Adhesion / drug effects
  • Cell Movement / drug effects
  • Female
  • Lung Neoplasms / secondary
  • Mice
  • Mice, Inbred BALB C
  • Neoplasm Transplantation
  • Phosphorylation
  • Protein Kinase C / drug effects
  • Protein Kinase C / metabolism
  • Sarcoma, Experimental / drug therapy*
  • Sarcoma, Experimental / enzymology
  • Sarcoma, Experimental / pathology*
  • Tetradecanoylphorbol Acetate / pharmacology*
  • Tumor Cells, Cultured / drug effects

Substances

  • Carcinogens
  • Protein Kinase C
  • Tetradecanoylphorbol Acetate