Does poly(ADP-ribosyl)ation regulate the DNA methylation pattern?

Biochemistry. 1997 Jul 1;36(26):7937-43. doi: 10.1021/bi970241s.


The existence of a possible correlation between poly(ADP-ribosyl)ation and DNA methylation processes was investigated. In vivo and in vitro experiments were carried out on L929 mouse fibroblasts preincubated for 24 h with or without 3-aminobenzamide, a well-known inhibitor of poly(ADP-ribose) polymerase. Both experimental approaches evidenced a close relationship between these two important nuclear enzymatic mechanisms, suggesting that the poly(ADP-ribosyl)ated isoform of H1 histone and/or long and branched protein-free ADP-ribose polymers could act as protecting agents against full methylation of the CpG dinucleotides in genomic DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Nucleus / metabolism
  • Cells, Cultured
  • DNA / metabolism*
  • DNA Methylation*
  • Fibroblasts / metabolism
  • Histones / metabolism
  • Mice
  • Poly Adenosine Diphosphate Ribose / metabolism*
  • Poly(ADP-ribose) Polymerases / metabolism


  • Histones
  • Poly Adenosine Diphosphate Ribose
  • DNA
  • Poly(ADP-ribose) Polymerases