Biomarker evidence of DNA oxidation in lung cancer patients: association of urinary 8-hydroxy-2'-deoxyguanosine excretion with radiotherapy, chemotherapy, and response to treatment

FEBS Lett. 1997 Jun 9;409(2):287-91. doi: 10.1016/s0014-5793(97)00523-1.

Abstract

Ratios of urinary 8-hydroxy-2'-deoxyguanosine to urinary creatinine (8-OHdG/creatinine) have been considered as a good biological indicator of DNA oxidation. Urinary 8-OHdG/creatinine levels of lung cancer patients were evaluated by enzyme-linked immunosorbent assay using a monoclonal antibody N45.1 during radiotherapy and chemotherapy. An increase in urinary 8-OHdG/creatinine was found in non-small-cell carcinoma (non-SCC) patients during the course of radiotherapy. SCC patients showed higher levels of urinary 8-OHdG/creatinine than the controls. Furthermore, SCC patients with complete or partial response to the chemotherapy showed a significant decrease in urinary 8-OHdG/creatinine while patients with no change or progressive disease showed an increase.

Publication types

  • Clinical Trial
  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers / urine
  • Carcinoma, Small Cell / drug therapy
  • Carcinoma, Small Cell / radiotherapy
  • Carcinoma, Small Cell / urine
  • Creatinine / urine
  • Cross-Sectional Studies
  • DNA / metabolism*
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / urine
  • Female
  • Humans
  • Lung Neoplasms / drug therapy
  • Lung Neoplasms / radiotherapy
  • Lung Neoplasms / urine*
  • Male
  • Middle Aged
  • Oxidation-Reduction
  • Smoking
  • Treatment Outcome

Substances

  • Biomarkers
  • 8-Hydroxy-2'-Deoxyguanosine
  • DNA
  • Creatinine
  • Deoxyguanosine