MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response

Mol Cell Endocrinol. 1997 May 16;129(2):229-35. doi: 10.1016/s0303-7207(97)04057-4.

Abstract

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated nuclear receptors that regulate transcription of target genes. Since attempts have been made to correlate the ingestion of high-fat diets, itself a peroxisome proliferator, with the occurrence of breast cancer, we set about to determine if human breast cancer cells contained a functional PPAR. In this report we demonstrate the presence of an mRNA in two breast cancer cell lines (MCF-7 and T47D) which is specifically recognized by a mouse PPARgamma2 probe. Furthermore, in gel shift assays a consensus PPAR response element (PPRE) was specifically bound by nuclear extracts from MCF-7 cells and was further retarded by antibodies raised to mouse PPARgamma. Finally, when transfected with a PPRE-luciferase transcriptional reporter construct, transcription was increased in response to activators of PPAR and its dimmeric partner the retinoic acid X receptor (RXR). These data indicate that peroxisomal proliferators are capable of mediating transcription in human breast cells and suggest the possibility of a physiological role in the breast.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology
  • Gene Expression Regulation
  • Humans
  • RNA, Messenger / analysis
  • Receptors, Cytoplasmic and Nuclear / genetics*
  • Transcription Factors / genetics*
  • Transcription, Genetic
  • Tumor Cells, Cultured

Substances

  • RNA, Messenger
  • Receptors, Cytoplasmic and Nuclear
  • Transcription Factors